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The microarray mRNA stability profiles confirmed that a number of transcripts could have improved fifty percent-lives while in the absence with the cytoplasmic LSM parts, AtLSM1 and AtLSM5, together with in plants lacking the nuclear AtLSM8 protein (Supplementary Desk S6).
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Edc3p and also a glutamine/asparagine-prosperous domain of Lsm4p operate in processing entire body assembly in Saccharomyces cerevisiae
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mutants resulted from a defect within their decapping, we checked the cap standing of selected stabilized mRNAs by immunoprecipitation with anti-cap antibodies (H20) accompanied by qRT-PCR to quantify the portion of immunoprecipitated transcripts. The amounts of capped mRNAs stabilized in lsm1a lsm1b
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